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Histone modification and signalling cascade of the dormancy‐associated MADS ‐box gene, PpMADS 13‐1 , in J apanese pear ( P yrus pyrifolia ) during endodormancy
Author(s) -
SAITO TAKANORI,
BAI SONGLING,
IMAI TSUYOSHI,
ITO AKIKO,
NAKAJIMA IKUKO,
MORIGUCHI TAKAYA
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12469
Subject(s) - mads box , histone , histone h4 , chromatin immunoprecipitation , biology , flowering locus c , chromatin , microbiology and biotechnology , histone h3 , gene , gene expression , genetics , promoter , mutant , repressor , arabidopsis
D ormancy‐associated MADS ‐box ( DAM ) genes play an important role in endodormancy phase transition. We investigated histone modification in the DAM homolog ( PpMADS 13‐1 ) from J apanese pear, via chromatin immunoprecipitation–quantitative PCR , to understand the mechanism behind the reduced expression of the PpMADS 13‐1 gene towards endodormancy release. Our results indicated that the reduction in the active histone mark by trimethylation of the histone H 3 tail at lysine 4 contributed to the reduction of PpMADS 13‐1 expression towards endodormancy release. In contrast, the inactive histone mark by trimethylation of the histone H 3 tail at lysine 27 in PpMADS 13‐1 locus was quite low, and these levels were more similar to a negative control [normal mouse immunoglobulin G ( IgG )] than to a positive control ( AGAMOUS ) in endodormancy phase transition. The loss of histone variant H2A.Z also coincided with the down‐regulation of PpMADS 13‐1 . Subsequently, we investigated the PpMADS 13‐1 signalling cascade and found that PpCBF 2, a pear C ‐repeated binding factor, regulated PpMADS 13‐1 expression via interaction of PpCBF 2 with the 5′‐upstream region of PpMADS 13‐1 by transient reporter assay. Furthermore, transient reporter assay confirmed no interaction between the PpMADS 13‐1 protein and the pear FLOWERING LOCUS T genes. Taken together, our results enhance understanding of the molecular mechanisms underlying endodormancy phase transition in J apanese pear.