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Chaperone network composition in S olanum lycopersicum explored by transcriptome profiling and microarray meta‐analysis
Author(s) -
FRAGKOSTEFANAKIS SOTIRIOS,
SIMM STEFAN,
PAUL PUNEET,
BUBLAK DANIELA,
SCHARF KLAUSDIETER,
SCHLEIFF ENRICO
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12426
Subject(s) - biology , transcriptome , heat shock protein , arabidopsis , gene , abiotic stress , microarray analysis techniques , gene expression profiling , heat shock , chaperone (clinical) , genetics , transcription factor , microbiology and biotechnology , gene expression , medicine , pathology , mutant
Heat shock proteins ( Hsps ) are molecular chaperones primarily involved in maintenance of protein homeostasis. Their function has been best characterized in heat stress ( HS ) response during which Hsps are transcriptionally controlled by HS transcription factors ( Hsfs ). The role of Hsfs and Hsps in HS response in tomato was initially examined by transcriptome analysis using the massive analysis of cDNA ends ( MACE ) method. Approximately 9.6% of all genes expressed in leaves are enhanced in response to HS , including a subset of Hsfs and Hsps . The underlying Hsp‐Hsf networks with potential functions in stress responses or developmental processes were further explored by meta‐analysis of existing microarray datasets. We identified clusters with differential transcript profiles with respect to abiotic stresses, plant organs and developmental stages. The composition of two clusters points towards two major chaperone networks. One cluster consisted of constitutively expressed plastidial chaperones and other genes involved in chloroplast protein homeostasis. The second cluster represents genes strongly induced by heat, drought and salinity stress, including HsfA 2 and many stress‐inducible chaperones, but also potential targets of HsfA 2 not related to protein homeostasis. This observation attributes a central regulatory role to HsfA 2 in controlling different aspects of abiotic stress response and tolerance in tomato.