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Quantitative trait loci and candidate genes underlying genotype by environment interaction in the response of A rabidopsis thaliana to drought
Author(s) -
ELSODA MOHAMED,
KRUIJER WILLEM,
MALOSETTI MARCOS,
KOORNNEEF MAARTEN,
AARTS MARK G. M.
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12418
Subject(s) - quantitative trait locus , biology , genetics , single nucleotide polymorphism , locus (genetics) , candidate gene , family based qtl mapping , population , arabidopsis , gene , arabidopsis thaliana , association mapping , drought tolerance , genetic linkage , genotype , inbred strain , gene mapping , chromosome , botany , mutant , demography , sociology
Drought stress was imposed on two sets of A rabidopsis thaliana genotypes grown in sand under short‐day conditions and analysed for several shoot and root growth traits. The response to drought was assessed for quantitative trait locus ( QTL ) mapping in a genetically diverse set of A rabidopsis accessions using genome‐wide association ( GWA ) mapping, and conventional linkage analysis of a recombinant inbred line ( RIL ) population. Results showed significant genotype by environment interaction ( G×E ) for all traits in response to different watering regimes. For the RIL population, the observed G×E was reflected in 17 QTL by environment interactions ( Q×E ), while 17 additional QTLs were mapped not showing Q×E . GWA mapping identified 58 single nucleotide polymorphism ( SNPs ) associated with loci displaying Q×E and an additional 16 SNPs associated with loci not showing Q×E . Many candidate genes potentially underlying these loci were suggested. The genes for RPS3C and YLS7 were found to contain conserved amino acid differences when comparing A rabidopsis accessions with strongly contrasting drought response phenotypes, further supporting their candidacy. One of these candidate genes co‐located with a QTL mapped in the RIL population.