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Identification of primary and secondary metabolites with phosphorus status‐dependent abundance in A rabidopsis , and of the transcription factor PHR 1 as a major regulator of metabolic changes during phosphorus limitation
Author(s) -
PANT BIKRAMDATT,
PANT POOJA,
ERBAN ALEXANDER,
HUHMAN DAVID,
KOPKA JOACHIM,
SCHEIBLE WOLFRÜDIGER
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12378
Subject(s) - primary metabolite , biochemistry , metabolic pathway , metabolism , secondary metabolism , mutant , biology , chemistry , enzyme , gene , biosynthesis
Massive changes in gene expression occur when plants are subjected to phosphorus ( P ) limitation, but the breadth of metabolic changes in these conditions and their regulation is barely investigated. Nearly 350 primary and secondary metabolites were profiled in shoots and roots of P ‐replete and P ‐deprived A rabidopsis thaliana wild type and mutants of the central P ‐signalling components PHR 1 and PHO 2, and micro RNA 399 overexpresser. In the wild type, the levels of 87 primary metabolites, including phosphorylated metabolites but not 3‐phosphoglycerate, decreased, whereas the concentrations of most organic acids, amino acids, nitrogenous compounds, polyhydroxy acids and sugars increased. Furthermore, the levels of 35 secondary metabolites, including glucosinolates, benzoides, phenylpropanoids and flavonoids, were altered during P limitation. Observed changes indicated P ‐saving strategies, increased photorespiration and crosstalk between P limitation and sulphur and nitrogen metabolism. The phr1 mutation had a remarkably pronounced effect on the metabolic P ‐limitation response, providing evidence that PHR 1 is a key factor for metabolic reprogramming during P limitation. The effects of pho2 or micro RNA 399 overexpression were comparatively minor. In addition, positive correlations between metabolites and gene transcripts encoding pathway enzymes were revealed. This study provides an unprecedented metabolic phenotype during P limitation in A rabidopsis .