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Inverse modulation of the energy sensor Snf 1‐related protein kinase 1 on hypoxia adaptation and salt stress tolerance in A rabidopsis thaliana
Author(s) -
IM JONG HEE,
CHO YOUNGHEE,
KIM GEUNDON,
KANG GEUNHO,
HONG JUNGWOO,
YOO SANGDONG
Publication year - 2014
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12375
Subject(s) - abscisic acid , crosstalk , microbiology and biotechnology , protein kinase a , biology , kinase , biochemistry , chemistry , gene , physics , optics
Terrestrial plants are exposed to complex stresses of high salt‐induced abscisic acid ( ABA ) and submergence‐induced hypoxia when seawater floods fields. Many studies have investigated plant responses to individual stress conditions, but not so much for coupled or sequentially imposed stresses. We examined molecular regulatory mechanisms of gene expression underlying the cellular responses involved in crosstalk between salt and hypoxia stresses. Salt/ ABA ‐ and AtMYC 2‐dependent induction of a synthetic ABA ‐responsive element and the native RD 22 promoters were utilized in our cell‐based functional assays. Such promoter‐based reporter induction was largely inhibited by hypoxia and hypoxia‐inducible AKIN 10 activity. Biochemical analyses showed that AKIN 10 negatively modulates AtMYC 2 protein accumulation via proteasome activity upon AKIN 10 kinase activity‐dependent protein modification. Further genetic analysis using transgenic plants expressing AKIN 10 provided evidence that AKIN 10 activity undermined AtMYC 2‐dependent salt tolerance. Our findings unravel a novel molecular interaction between the key signalling constituents leading crosstalk between salt and hypoxia stresses in A rabidopsis thaliana under the detrimental condition of submergence in saltwater.