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Identification of quantitative trait loci and a candidate locus for freezing tolerance in controlled and outdoor environments in the overwintering crucifer B oechera stricta
Author(s) -
HEO JAEYUN,
FENG DONGSHENG,
NIU XIAOMU,
MITCHELLOLDS THOMAS,
TIENDEREN PETER H.,
TOMES DWIGHT,
SCHRANZ M. ERIC
Publication year - 2014
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12365
Subject(s) - quantitative trait locus , biology , locus (genetics) , inbred strain , genetics , photosynthesis , overwintering , drought tolerance , botany , horticulture , gene
Development of chilling and freezing tolerance is complex and can be affected by photoperiod, temperature and photosynthetic performance; however, there has been limited research on the interaction of these three factors. We evaluated 108 recombinant inbred lines of B oechera stricta , derived from a cross between lines originating from M ontana and C olorado, under controlled long day ( LD ), short‐day ( SD ) and in an outdoor environment ( OE ). We measured maximum quantum yield of photosystem II , lethal temperature for 50% survival and electrolyte leakage of leaves. Our results revealed significant variation for chilling and freezing tolerance and photosynthetic performance in different environments. Using both single‐ and multi‐trait analyses, three main‐effect quantitative trait loci ( QTL ) were identified. QTL on linkage group ( LG )3 were SD specific, whereas QTL on LG 4 were found under both LD and SD . Under all conditions, QTL on LG 7 were identified, but were particularly predictive for the outdoor experiment. The co‐localization of photosynthetic performance and freezing tolerance effects supports these traits being co‐regulated. Finally, the major QTL on LG 7 is syntenic to the A rabidopsis C ‐repeat binding factor locus, known regulators of chilling and freezing responses in A rabidopsis thaliana and other species.