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Magnesium availability regulates the development of root hairs in A rabidopsis thaliana ( L .) H eynh
Author(s) -
NIU YAOFANG,
CHAI RUSHAN,
LIU LIJUAN,
JIN GULEI,
LIU MIAO,
TANG CAIXIAN,
ZHANG YONGSONG
Publication year - 2014
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12362
Subject(s) - root hair , elongation , arabidopsis thaliana , cytosol , chemistry , reactive oxygen species , microbiology and biotechnology , transcriptome , biochemistry , magnesium , botany , mutant , biophysics , biology , gene , gene expression , materials science , organic chemistry , metallurgy , ultimate tensile strength , enzyme
Root hairs are reported to be plastic in response to nutrient supply, but relatively little is known about their development in response to magnesium ( Mg ) availability. Here, we showed that development of root hairs of A rabidopsis decreased progressively with increasing Mg supply, which was related to the initiation of new trichoblast files and likelihood of trichoblasts to form hairs. Tip‐focused reactive oxygen species ( ROS ) and cytosolic Ca 2+ concentrations [( Ca 2+ )c] during elongation of root hairs were enhanced under low Mg but decreased under high Mg . Under low Mg , application of diphenylene iodonium (DPI ) or BAPTA [1,2‐bis( o ‐aminophenoxy)ethane‐ N , N , N ′, N ′‐tetraacetic acid] blocked the enhanced development of root hairs and the opposite was true when the plants under high Mg were treated with phenazine methosulphate ( PMS ), methyl viologen ( MV ) or CaCl 2 . Furthermore, Mg availability did not alter root hair growth in rhd2‐1 mutant that contains lower levels of ROS and cytosolic [ Ca 2+ ]c. Transcriptome data and q PCR results revealed a greater fraction of morphogenetic H ‐genes, and cell wall organization genes were up‐regulated by low Mg but down‐regulated by high Mg . Our data suggest a profound effect of Mg supply on the development of root hairs in A rabidopsis , through the characterized Ca 2+ and ROS signals that modulate the elongation of root hairs and the expression of root‐hair morphogenetic genes.