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Banana fruit NAC transcription factor MaNAC 1 is a direct target of MaICE 1 and involved in cold stress through interacting with MaCBF 1
Author(s) -
SHAN WEI,
KUANG JIANFEI,
LU WANGJIN,
CHEN JIANYE
Publication year - 2014
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12303
Subject(s) - transcription factor , microbiology and biotechnology , chemistry , biology , biochemistry , gene
Our previous studies have indicated that the banana ripening‐induced MaNAC1 , a NAC ( NAM , ATAF 1/2 and CUC 2) transcription factor ( TF ) gene, is regulated by ethylene during fruit ripening, and propylene, a functional ethylene analogue, induces cold tolerance of banana fruits. However, the involvement of MaNAC1 in propylene‐induced cold tolerance of banana fruits is not understood. In the present work, the possible involvement of MaNAC 1 in cold tolerance of banana fruits was investigated. MaNAC1 was noticeably induced by cold stress or following propylene treatment during cold storage. Transient protoplast assays showed that MaNAC1 promoter was activated by cold stress and ethylene treatment. Yeast one‐hybrid ( Y 1 H ), electrophoretic mobility shift assay ( EMSA ) and transient expression assays demonstrated MaNAC1 as a novel direct target of MaICE 1, and that the ability of MaICE 1 binding to MaNAC1 promoter might be enhanced by MaICE 1 phosphorylation and cold stress. Moreover, yeast two‐hybrid ( Y 2 H ) and bimolecular fluorescence complementation ( BiFC ) analyses revealed physical interaction between MaNAC 1 and MaCBF 1, a downstream component of inducer of C‐repeat binding factor (CBF) expression 1 ( ICE 1) in cold signalling. Taken together, these results suggest that the cold‐responsive MaNAC1 may be involved in cold tolerance of banana fruits through its interaction with ICE 1– CBF cold signalling pathway, providing new insights into the regulatory activity of NAC TF .