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LESION SIMULATING DISEASE 1 and ENHANCED DISEASE SUSCEPTIBILITY 1 differentially regulate UV ‐ C ‐induced photooxidative stress signalling and programmed cell death in A rabidopsis thaliana
Author(s) -
WITUSZYŃSKA WERONIKA,
SZECHYŃSKAHEBDA MAGDALENA,
SOBCZAK MIROSŁAW,
RUSACZONEK ANNA,
KOZŁOWSKAMAKULSKA ANNA,
WITOŃ DAMIAN,
KARPIŃSKI STANISŁAW
Publication year - 2015
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12288
Subject(s) - programmed cell death , microbiology and biotechnology , reactive oxygen species , biology , oxidative stress , apoptosis , signal transduction , chloroplast , organelle , regulator , obligate , biophysics , biochemistry , botany , gene
As obligate photoautotrophs, plants are inevitably exposed to ultraviolet ( UV ) radiation. Because of stratospheric ozone depletion, UV has become more and more dangerous to the biosphere. Therefore, it is important to understand UV perception and signal transduction in plants. In the present study, we show that LESION SIMULATING DISEASE 1 ( LSD 1) and ENHANCED DISEASE SUSCEPTIBILITY 1 ( EDS 1) are antagonistic regulators of UV ‐ C ‐induced programmed cell death ( PCD ) in A rabidopsis thaliana . This regulatory dependence is manifested by a complex deregulation of photosynthesis, reactive oxygen species homeostasis, antioxidative enzyme activity and UV ‐responsive genes expression. We also prove that a UV ‐ C radiation episode triggers apoptotic‐like morphological changes within the mesophyll cells. Interestingly, chloroplasts are the first organelles that show features of UV ‐ C ‐induced damage, which may indicate their primary role in PCD development. Moreover, we show that A rabidopsis Bax inhibitor 1 ( AtBI 1), which has been described as a negative regulator of plant PCD , is involved in LSD 1‐dependent cell death in response to UV ‐ C . Our results imply that LSD 1 and EDS 1 regulate processes extinguishing excessive energy, reactive oxygen species formation and subsequent PCD in response to different stresses related to impaired electron transport.