Premium
Cadmium induces two waves of reactive oxygen species in G lycine max ( L .) roots
Author(s) -
PÉREZCHACA MARÍA VERÓNICA,
RODRÍGUEZSERRANO MARÍA,
MOLINA ALICIA S.,
PEDRANZANI HILDA E.,
ZIRULNIK FANNY,
SANDALIO LUISA M.,
ROMEROPUERTAS MARÍA C.
Publication year - 2014
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12280
Subject(s) - cadmium , chemistry , oxygen , reactive oxygen species , physics , food science , biochemistry , organic chemistry
Cadmium ( Cd ) is a non‐essential heavy metal that may be toxic or even lethal to plants as it can be easily taken up by the roots and loaded into the xylem to the leaves. Using soybean roots ( G lycine max L. ) DM 4800, we have analysed various parameters related to reactive oxygen metabolism and nitric oxide ( NO ) during a 6 day Cd exposure. A rise in H 2 O 2 and NO , and to a lesser extent O 2 ·− content was observed after 6 h exposure with a concomitant increase in lipid peroxidation and carbonyl group content. Both oxidative markers were significantly reduced after 24 h. A second, higher wave of O 2 ·− production was also observed after 72 h of exposure followed by a reduction until the end of the treatment. NOX and glicolate oxidase activity might be involved in the initial Cd ‐induced reactive oxygen species (ROS) production and it appears that other sources may also participate. The analysis of antioxidative enzymes showed an increase in glutathione‐ S ‐transferase activity and in transcript levels and activity of enzymes involved in the ascorbate‐glutathione cycle and the NADPH ‐generating enzymes. These results suggest that soybean is able to respond rapidly to oxidative stress imposed by Cd by improving the availability of NADPH necessary for the ascorbate‐glutathione cycle.