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Mutants of phospholipase A ( pPLA ‐ I ) have a red light and auxin phenotype
Author(s) -
EFFENDI YUNUS,
RADATZ KATRIN,
LABUSCH CORINNA,
RIETZ STEFFEN,
WIMALASEKERA RINUKSHI,
HELIZON HANNA,
ZEIDLER MATHIAS,
SCHERER GÜNTHER F. E.
Publication year - 2014
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12278
Subject(s) - auxin , phototropism , phytochrome , mutant , gravitropism , biology , microbiology and biotechnology , phenotype , shade avoidance , arabidopsis , wild type , phospholipase , phytochrome a , biochemistry , gene , botany , enzyme , red light , blue light , physics , optics
pPLA‐I is the evolutionarily oldest patatin‐related phospholipase A ( pPLA ) in plants, which have previously been implicated to function in auxin and defence signalling. Molecular and physiological analysis of two allelic null mutants for pPLA‐I [ ppla‐I‐1 in W assilewskija ( W s) and ppla‐I‐3 in C olumbia ( C ol) ] revealed pPLA‐I functions in auxin and light signalling. The enzyme is localized in the cytosol and to membranes. After auxin application expression of early auxin‐induced genes is significantly slower compared with wild type and both alleles show a slower gravitropic response of hypocotyls, indicating compromised auxin signalling. Additionally, phytochrome‐modulated responses like abrogation of gravitropism, enhancement of phototropism and growth in far red‐enriched light are decreased in both alleles. While early flowering, root coils and delayed phototropism are only observed in the Ws mutant devoid of phyD , the light‐related phenotypes observed in both alleles point to an involvement of pPLA‐I in phytochrome signalling.