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ARP 2/3 complex‐mediated actin dynamics is required for hydrogen peroxide‐induced stomatal closure in A rabidopsis
Author(s) -
LI XIN,
LI JIANHUA,
WANG WEI,
CHEN NAIZHI,
MA TONGSUO,
XI YANAN,
ZHANG XIAOLU,
LIN HAIFEI,
BAI YANG,
HUANG SHANJIN,
CHEN YULING
Publication year - 2014
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12259
Subject(s) - guard cell , abscisic acid , microbiology and biotechnology , actin , actin cytoskeleton , arabidopsis , chemistry , cytoskeleton , biology , mutant , biochemistry , cell , gene
Multiple cellular events like dynamic actin reorganization and hydrogen peroxide ( H 2 O 2 ) production were demonstrated to be involved in abscisic acid ( ABA )‐induced stomatal closure. However, the relationship between them as well as the underlying mechanisms remains poorly understood. Here, we showed that H 2 O 2 generation is indispensable for ABA induction of actin reorganization in guard cells of Arabidopsis that requires the presence of ARP 2/3 complex. H 2 O 2 ‐induced stomatal closure was delayed in the mutants of arpc4 and arpc5 , and the rate of actin reorganization was slowed down in arpc4 and arpc5 in response to H 2 O 2 , suggesting that ARP 2/3‐mediated actin nucleation is required for H 2 O 2 ‐induced actin cytoskeleton remodelling. Furthermore, the expression of H 2 O 2 biosynthetic related gene AtrbohD and the accumulation of H 2 O 2 was delayed in response to ABA in arpc4 and arpc5 , demonstrating that misregulated actin dynamics affects H 2 O 2 production upon ABA treatment. These results support a possible causal relation between the production of H 2 O 2 and actin dynamics in ABA ‐mediated guard cell signalling: ABA triggers H 2 O 2 generation that causes the reorganization of the actin cytoskeleton partially mediated by ARP 2/3 complex, and ARP 2/3 complex‐mediated actin dynamics may feedback regulate H 2 O 2 production.