ARP 2/3 complex‐mediated actin dynamics is required for hydrogen peroxide‐induced stomatal closure in  A rabidopsis | Zendy

LI XIN | Zendy; LI JIANHUA | Zendy; WANG WEI | Zendy; CHEN NAIZHI | Zendy; MA TONGSUO | Zendy; XI YANAN | Zendy; ZHANG XIAOLU | Zendy; LIN HAIFEI | Zendy; BAI YANG | Zendy; HUANG SHANJIN | Zendy; CHEN YULING | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

ARP 2/3 complex‐mediated actin dynamics is required for hydrogen peroxide‐induced stomatal closure in A rabidopsis

Author(s) -

LI XIN,

LI JIANHUA,

WANG WEI,

CHEN NAIZHI,

MA TONGSUO,

XI YANAN,

ZHANG XIAOLU,

LIN HAIFEI,

BAI YANG,

HUANG SHANJIN,

CHEN YULING

Publication year - 2014

Publication title -

plant, cell and environment

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.646

H-Index - 200

eISSN - 1365-3040

pISSN - 0140-7791

DOI - 10.1111/pce.12259

Subject(s) - guard cell , abscisic acid , microbiology and biotechnology , actin , actin cytoskeleton , arabidopsis , chemistry , cytoskeleton , biology , mutant , biochemistry , cell , gene

Multiple cellular events like dynamic actin reorganization and hydrogen peroxide ( H 2 O 2 ) production were demonstrated to be involved in abscisic acid ( ABA )‐induced stomatal closure. However, the relationship between them as well as the underlying mechanisms remains poorly understood. Here, we showed that H 2 O 2 generation is indispensable for ABA induction of actin reorganization in guard cells of Arabidopsis that requires the presence of ARP 2/3 complex. H 2 O 2 ‐induced stomatal closure was delayed in the mutants of arpc4 and arpc5 , and the rate of actin reorganization was slowed down in arpc4 and arpc5 in response to H 2 O 2 , suggesting that ARP 2/3‐mediated actin nucleation is required for H 2 O 2 ‐induced actin cytoskeleton remodelling. Furthermore, the expression of H 2 O 2 biosynthetic related gene AtrbohD and the accumulation of H 2 O 2 was delayed in response to ABA in arpc4 and arpc5 , demonstrating that misregulated actin dynamics affects H 2 O 2 production upon ABA treatment. These results support a possible causal relation between the production of H 2 O 2 and actin dynamics in ABA ‐mediated guard cell signalling: ABA triggers H 2 O 2 generation that causes the reorganization of the actin cytoskeleton partially mediated by ARP 2/3 complex, and ARP 2/3 complex‐mediated actin dynamics may feedback regulate H 2 O 2 production.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research