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Effects of water availability on emerald ash borer larval performance and phloem phenolics of Manchurian and black ash
Author(s) -
CHAKRABORTY SOURAV,
WHITEHILL JUSTIN G. A.,
HILL AMY L.,
OPIYO STEPHEN O.,
CIPOLLINI DON,
HERMS DANIEL A.,
BONELLO PIERLUIGI
Publication year - 2014
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12215
Subject(s) - pinoresinol , phloem , emerald ash borer , larva , fraxinus , biology , botany , raphanus , lignan
The invasive emerald ash borer ( EAB ) beetle is a significant threat to the survival of N orth A merican ash. In previous work, we identified putative biochemical and molecular markers of constitutive EAB resistance in M anchurian ash, an A sian species co‐evolved with EAB . Here, we employed high‐throughput high‐performance liquid chromatography with photodiode array detection and mass spectrometry ( HPLC‐PDA ‐ MS) to characterize the induced response of soluble phloem phenolics to EAB attack in resistant M anchurian and susceptible black ash under conditions of either normal or low water availability, and the effects of water availability on larval performance. Total larval mass per tree was lower in M anchurian than in black ash. Low water increased larval numbers and mean larval mass overall, but more so in M anchurian ash. Low water did not affect levels of phenolics in either host species, but six phenolics decreased in response to EAB . In both ashes, pinoresinol A was induced by EAB , especially in M anchurian ash. Pinoresinol A and pinoresinol B were negatively correlated with each other in both species. The higher accumulation of pinoresinol A in M anchurian ash after attack may help explain the resistance of this species to EAB , but none of the responses measured here could explain increased larval performance in trees subjected to low water availability.