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Cryptochrome 1 regulates growth and development in B rassica through alteration in the expression of genes involved in light, phytohormone and stress signalling
Author(s) -
SHARMA POOJA,
CHATTERJEE MITHU,
BURMAN NAINI,
KHURANA JITENDRA P.
Publication year - 2014
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12212
Subject(s) - photomorphogenesis , cryptochrome , abscisic acid , biology , hypocotyl , microbiology and biotechnology , abiotic stress , arabidopsis , etiolation , auxin , transcriptome , botany , abiotic component , raphanus , biotic stress , gene , gene expression , genetics , circadian clock , biochemistry , paleontology , mutant , enzyme
Abstract The blue light photoreceptors cryptochromes are ubiquitous in higher plants and are vital for regulating plant growth and development. In spite of being involved in controlling agronomically important traits like plant height and flowering time, cryptochromes have not been extensively characterized from agriculturally important crops. Here we show that overexpression of CRY1 from B rassica napus ( BnCRY1 ), an oilseed crop, results in short‐statured B rassica transgenics, likely to be less prone to wind and water lodging. The overexpression of BnCRY1 accentuates the inhibition of cell elongation in hypocotyls of transgenic seedlings. The analysis of hypocotyl growth inhibition and anthocyanin accumulation responses in BnCRY1 overexpressors substantiates that regulation of seedling photomorphogenesis by cry1 is dependent on light intensity. This study highlights that the photoactivated cry1 acts through coordinated induction and suppression of specific downstream genes involved in phytohormone synthesis or signalling, and those involved in cell wall modification, during de‐etiolation of B rassica seedlings. The microarray‐based transcriptome profiling also suggests that the overexpression of BnCRY1 alters abiotic/biotic stress signalling pathways; the transgenic seedlings were apparently oversensitive to abscisic acid ( ABA ) and mannitol.