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A new link between stress response and nucleolar function during pollen development in A rabidopsis mediated by AtREN 1 protein
Author(s) -
REŇÁK DAVID,
GIBALOVÁ ANTÓNIA,
ŠOLCOVÁ KATARZYNA,
HONYS DAVID
Publication year - 2014
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12186
Subject(s) - biology , gametophyte , pollen , nucleolus , genetics , gene , heat shock factor , heat shock protein , microbiology and biotechnology , transcription factor , heat shock , mutant , botany , hsp70 , nucleus
Heat shock transcription factors ( H sfs) are involved in multiple aspects of stress response and plant growth. However, their role during male gametophyte development is largely unknown, although the generative phase is the most sensitive and critical period in the plant life cycle. Based on a wide screen of T ‐ DNA mutant lines, we identified the atren1 mutation ( restricted to nucleolus1 ) in early male gametophytic gene A t1g77570, which has the closest homology to HSFA 5 gene, the member of a heat shock transcription factor ( HSF ) gene family. The mutation causes multiple defects in male gametophyte development in both structure and function. Because the mutation disrupts an early acting ( AtREN 1 ) gene, these pollen phenotype abnormalities appear from bicellular pollen stage to pollen maturation. Moreover, the consequent progamic phase is compromised as well as documented by pollen germination defects and limited transmission via male gametophyte. In addition, atren1/− plants are defective in heat stress ( HS ) response and produce notably higher proportion of aberrant pollen grains. AtREN 1 protein is targeted specifically to the nucleolus that, together with the increased size of the nucleolus in atren1 pollen, suggests that it is likely to be involved in ribosomal RNA biogenesis or other nucleolar functions.