Premium
Cold‐induced organelle relocation in the liverwort M archantia polymorpha L.
Author(s) -
OGASAWARA YUKA,
ISHIZAKI KIMITSUNE,
KOHCHI TAKAYUKI,
KODAMA YUTAKA
Publication year - 2013
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12085
Subject(s) - organelle , marchantia polymorpha , chloroplast , biology , cell wall , microbiology and biotechnology , cell , botany , biochemistry , gene
Organelles change their subcellular positions in response to various environmental conditions. Recently, we reported that cold treatments alter the intracellular position of chloroplasts and nuclei (cold positioning) in the fern A diantum capillus‐veneris ; chloroplasts and nuclei localized to the periclinal cell wall relocated to anticlinal cell wall after cold treatments. To further understand organelle positioning under cold conditions, we studied cold‐induced organelle relocation in the liverwort M archantia polymorpha L . When sporelings and gemmmalings were treated under low temperature (5 °C), chloroplast cold positioning response was successfully induced both in the sporelings and the gemmmalings of M . polymorpha . Using a genetic transformation, nuclei, mitochondria or peroxisomes were visualized with a fluorescent protein, and the transgenic gemmmalings were incubated under the cold condition. Nuclei and peroxisomes, but not mitochondria, clearly relocated from the periclinal cell wall to the anticlinal cell wall after cold treatments. Our findings suggest that several organelles concurrently change their positions in the liverwort cell to cope with cold temperature.