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The response of A sterochloris erici ( A hmadjian) S kaloud et P eksa to desiccation: a proteomic approach
Author(s) -
GASULLA FRANSCICO,
JAIN RENUKA,
BARRENO EVA,
GUÉRA ALFREDO,
BALBUENA TIAGO S.,
THELEN JAY J.,
OLIVER MELVIN J.
Publication year - 2013
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12065
Subject(s) - desiccation , desiccation tolerance , dehydration , proteome , proteomics , biology , biochemistry , oxidative stress , microbiology and biotechnology , botany , gene
The study of desiccation tolerance of lichens, and of their chlorobionts in particular, has frequently focused on the antioxidant system that protects the cell against photo‐oxidative stress during dehydration/rehydration cycles. In this study, we used proteomic and transcript analyses to assess the changes associated with desiccation in the isolated phycobiont A sterochloris erici . Algae were dried either slowly (5–6 h) or rapidly (<60 min), and rehydrated after 24 h in the desiccated state. To identify proteins that accumulated during the drying or rehydration processes, we employed two‐dimensional (2 D ) difference gel electrophoresis ( DIGE ) coupled with individual protein identification using trypsin digestion and liquid chromatography‐tandem mass spectrometry ( LC‐MS / MS ). Proteomic analyses revealed that desiccation caused an increase in relative abundance of only 11–13 proteins, regardless of drying rate, involved in glycolysis, cellular protection, cytoskeleton, cell cycle, and targeting and degradation. Transcripts of five H sp90 and two β‐tubulin genes accumulated primarily at the end of the dehydration process. In addition, transmission electron microscopy ( TEM ) images indicate that ultrastructural cell injuries, perhaps resulting from physical or mechanical stress rather than metabolic damage, were more intense after rapid dehydration. This occurred with no major change in the proteome. These results suggest that desiccation tolerance of A. erici is achieved by constitutive mechanisms.