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Down‐regulation of catalase activity allows transient accumulation of a hydrogen peroxide signal in C hlamydomonas reinhardtii
Author(s) -
MICHELET LAURE,
ROACH THOMAS,
FISCHER BEAT B.,
BEDHOMME MARIETTE,
LEMAIRE STÉPHANE D.,
KRIEGERLISZKAY ANJA
Publication year - 2013
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12053
Subject(s) - catalase , chlamydomonas reinhardtii , hydrogen peroxide , reactive oxygen species , biophysics , oxidative stress , chemistry , biochemistry , chloroplast , microbiology and biotechnology , photoprotection , photosynthesis , cytosol , enzyme , mutant , biology , gene
We provide evidence that a floodgate mechanism of ROS signalling that had been proposed to occur in mammalian cells may also take place in C hlamydomonas reinhardtii . When cells of C . reinhardtii were shifted from dark to high light, a transient increase in the level of hydrogen peroxide was observed which correlated with a reversible partial inactivation of catalase activity. This transient increase in the H 2 O 2 level seems to allow to activate H 2 O 2 ‐dependent signaling pathways since changes in the expression level of H 2 O 2 responsive genes such as HSP 22C tightly followed the peak of H 2 O 2 .