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Overexpression of the phosphatidylinositol synthase gene ( ZmPIS ) conferring drought stress tolerance by altering membrane lipid composition and increasing ABA synthesis in maize
Author(s) -
LIU XIUXIA,
ZHAI SHUMEI,
ZHAO YAJIE,
SUN BAOCHENG,
LIU CHENG,
YANG AIFANG,
ZHANG JUREN
Publication year - 2013
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12040
Subject(s) - gene , genetically modified crops , biosynthesis , transgene , genetically modified maize , biology , galactolipids , phosphatidylinositol , function (biology) , microbiology and biotechnology , biochemistry , botany , signal transduction , chloroplast
In this study, the ZmPIS gene with a maize ubiquitin promoter was introduced into maize (Zea mays L.) inbred line DH4866 by an Agrobacterium‐mediated method to explore the function of the ZmPIS gene in the response of maize to drought stress. The overexpression of ZmPIS in maize resulted in significantly elevated levels of most phospholipids, galactolipids in leaves compared with those in WT and markedly up‐regulated expression of some genes involved in the phospholipids metabolism pathway and the ABA biosynthesis pathway after drought stress. Consistent with these results, the drought stress tolerance of the ZmPIS sense transgenic plants were enhanced significantly compared to WT maize plants. These results imply that ZmPIS regulate the plant response to drought stress through altering membrane lipid composition and increasing ABA synthesis in maize.