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T oc33 and T oc64‐ III cooperate in precursor protein import into the chloroplasts of A rabidopsis thaliana
Author(s) -
SOMMER MANUEL,
RUDOLF MAREIKE,
TILLMANN BODO,
TRIPP JOANNA,
SOMMER MAIK S.,
SCHLEIFF ENRICO
Publication year - 2013
Publication title -
plant, cell and environment
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.646
H-Index - 200
eISSN - 1365-3040
pISSN - 0140-7791
DOI - 10.1111/pce.12030
Subject(s) - arabidopsis thaliana , chloroplast , mutant , arabidopsis , microbiology and biotechnology , function (biology) , biology , chemistry , biochemistry , botany , gene
It has been established that the TOC components T oc159, T oc75 and Toc33 play crucial roles in chloroplast preprotein import, while the importance of T oc64‐ III for TOC function remained under debate. To link an involvement of T oc64‐ III to preprotein import and to the action of Toc33, single and double mutants thereof have been generated and phenotypically analyzed and the import capacity has been monitored in vivo . These experiments confirm that T oc64‐ III is required for preprotein import. In addition, the double mutant of toc64‐ III and toc33 shows a significantly reduced level of Toc75‐ III demonstrating that both TOC components act cooperatively for the insertion of at least this outer membrane protein.