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A multiplex PCR marker distinguishes between a series of four LanFTc1 alleles regulating flowering time in narrow‐leafed lupin ( Lupinus angustifolius )
Author(s) -
Taylor Candy M.,
Zanden Julian,
Saradadevi Renu,
Berger Jens D.,
Kamphuis Lars G.,
Pradhan Aneeta,
Sharma Darshan,
Nelson Matthew N.,
Cowling Wallace A.
Publication year - 2021
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12982
Subject(s) - biology , lupinus angustifolius , indel , allele , genetics , indel mutation , introgression , genotype , multiplex , gene , botany , single nucleotide polymorphism
We designed and validated a new multiplex PCR marker which discriminates between four insertion/deletion (INDEL) alleles in the 5′ regulatory region of a major flowering time gene in Lupinus angustifolius , LanFTc1 . The four INDEL alleles were the wild‐type allele ( ku ) in variety ‘Geebung’ (G), a 1,208‐bp deletion allele in accession P22660 (P), a 1,423‐bp deletion allele ( Ku ) in variety ‘Tanjil’ (T) and a 5,162‐bp deletion allele ( Jul ) in variety ‘Krasnolistny’ (K). F 2 PCR marker genotypes segregated as expected in populations K × G, K × T, P × G and P × T. Heritability of flowering times based on F 2:3 parent:offspring correlation was high in K × G (0.81 ± 0.09), P × G (0.76 ± 0.10) and P × T (0.81 ± 0.11) but low in K × T (−0.04 ± 0.15) due to similar early‐flowering phenotypes produced by Ku and Jul . Progeny homozygous for the 1,208‐bp deletion allele resulted in a unique array of mid‐season phenology in the F 2 , F 3 and F 4 generations, which may improve agronomic adaptation. This multiplex PCR marker will improve the efficiency of introgression of the new INDELs into future lupin varieties.