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Genetics and molecular markers for anthocyanin pigmentation in barnyard millet ( Echinochloa frumentacea (Roxb.) Link)
Author(s) -
Renganathan Vellaichamy Gandhimeyyan,
Vanniarajan Chockalingam,
Senthil Natesan,
Nirmalakumari Angamuthu,
Karthikeyan Adhimoolam,
Veni Kannan,
Ramalingam Jegadeesan
Publication year - 2021
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12892
Subject(s) - biology , anthocyanin , inflorescence , echinochloa , pigment , population , genotype , botany , genetic marker , genetics , gene , weed , chemistry , sociology , demography , organic chemistry
This study aimed to track the inheritance and identify the expressed sequence tag (EST) ‐ simple sequence repeat (SSR) marker (s) associated with the anthocyanin pigments in barnyard millet ( Echinochloa spp.). The F 1 and F 2 generations were developed using the anthocyanin pigmented genotype ACM 331 (purple inflorescence) and three non‐pigmented genotypes, ACM 333, ACM 335 and MA 10 (green inflorescence). The F 1 plants of all the three crosses had medium purple inflorescence indicating that purple pigment was partially dominant over green inflorescence. The segregation ratio of F 2 generation is 1:2:1 (purple:medium purple:green) further confirmed that purple is partially dominant over the green and governed by one gene. Fifty‐one EST‐SSR markers were used to screen the pigmented and non‐pigmented bulks of the F 2 population. A marker, BMESSR 39 associated with the anthocyanin pigment trait was obtained, which revealed a high correlation with the anthocyanin pigment present in the F 2 population consisting of 430 individuals (85.10%) and 80 barnyard millet genotypes (63.75%). The information obtained in this study could pave way for breeding anthocyanin pigmented genotypes and genetic studies in barnyard millet.