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Construction of high‐density linkage map and identification of QTLs associated with resistance to black rot in radish ( Raphanus sativus ) by RAD sequencing
Author(s) -
Wu Chunhui,
Qiu Yang,
Duan Yundan,
Guo Yangdong,
Wang Haiping,
Zhang Xiaohui,
Song Jiangping,
Li Xixiang
Publication year - 2020
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12794
Subject(s) - biology , indel , quantitative trait locus , raphanus , genetics , genetic linkage , population , linkage (software) , genotyping , genetic marker , gene mapping , plant disease resistance , snp genotyping , single nucleotide polymorphism , genotype , gene , horticulture , chromosome , demography , sociology
High‐density marker‐based QTL mapping can serve as an effective strategy to identify novel genomic information to facilitate crop improvement. In this study, we genotyped an F 2 population (KB12‐1 × PP12‐1) using a RAD‐seq approach and constructed a high‐density linkage map for radish. After a series of filtering procedures were performed, 17,124 SNPs and 3,336 indels with aa × bb genotyping were retained to obtain bin markers. Then, a linkage map comprising a total of 1,221 bin markers in nine linkage groups spanning 1,467.3 cM with an average marker interval of 1.2 cM was constructed. We evaluated the resistance of the F 2 mapping population to black rot using F 3 progeny, and two major QTLs related to black rot resistance were identified based on this map. Among these QTLs, qBRR2 on Chr.2 explained 26.97% of the phenotypic variation with a LOD score of 11.93, and qBRR7 on Chr.7 accounted for 27.06% of the phenotypic variation with a LOD score of 11.83. The additive effect of qBRR2 was positive (14.97); however, qBRR7 had the opposite effect (−11.99). The high‐density linkage map and the major QTLs qBRR2 and qBRR7 provide new important information for disease resistance gene discovery and utilization in genetic improvement.