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Development of a large population of activation‐tagged mutants in an elite indica rice variety
Author(s) -
Kota Vamsee Raja,
Gundra Sivakrishna Rao,
Vudem Dashavantha Reddy,
Pulugurtha Bharadwaja Kirti,
Khareedu Venkateswara Rao
Publication year - 2020
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12780
Subject(s) - biology , mutant , panicle , genetically modified rice , population , hygromycin b , oryza sativa , germination , botany , gene , transgene , genetically modified crops , agronomy , horticulture , genetics , demography , sociology
In this study, we have generated more than 12,000 activation‐tagged mutants in a high‐yielding indica rice variety, 'BPT 5204', employing maize Ac/Ds system. Different transgenic plants obtained were analysed based on expression patterns of green fluorescence protein (GFP), red fluorescence protein (RFP), herbicide (Basta) tolerance and molecular analyses. T 1 seeds of pSQ5 and pSQ5‐ bar transgenics, when germinated separately on hygromycin (50 mg/L) and phosphinothricin (5 mg/L) containing medium, revealed a segregation of 3 tolerant : 1 susceptible plants. The germinal transposition frequency of Ds element in different T 2 progeny of rice plants was found to be about 18.0%. Different stable tagged mutants exhibited marked increases in plant height, number of tillers, leaf size, panicle size, seed size and number of grains per plant. The overall results indicate that the genes associated with these traits are upregulated by the enhancer element in activation‐tagged mutants. As such, the various tagged mutant lines appear promising and serve as a valuable genetic resource for identification of key genes determining different agronomic traits of rice.