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The possible role of BnaA10.SOI.a in seed fatty acid biosynthesis of rapeseed
Author(s) -
Gong Jingyun,
Li Dong,
Li Xinye,
Yu Xuchen,
Guo Yuan,
Chen Mingxun
Publication year - 2020
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12766
Subject(s) - myb , brassica , biology , arabidopsis , mutant , rapeseed , ectopic expression , arabidopsis thaliana , transcription factor , gene , wild type , microbiology and biotechnology , botany , biochemistry , genetics
In Arabidopsis thaliana , AtMYB76 is an MYB transcription factor that has been shown to play multiple roles in the biosynthesis of aliphatic glucosinolates and fatty acids. However, in Brassica napus , the role of BnaA10.SOI.a , an AtMYB76 orthologue, in the accumulation of seed lipids remains unclear. In the present study, we obtained the BnaA10.SOI.a coding domain sequence from the Brassica napus cultivar 'Zhongshuang 11' and analysed its conserved protein domain. A subcellular localization experiment in tobacco leaf cells indicated that BnaA10.SOI.a functioned as a transcription factor. Our results demonstrated that the introduction of BnaA10.SOI.a into an Arabidopsis thaliana myb76‐2 mutant could restore wild‐type levels of seed fatty acid content. Moreover, the expression levels of six oil biosynthetic genes in developing seeds of myb76‐2 were restored to wild‐type levels by ectopic expression of BnaA10.SOI.a . These results improve our understanding of the function of BnaA10.SOI.a and provide a potential target for genetic manipulations of B. napus to improve oil quantity in seeds.