Premium
Genetic dissection of vitamin E content in rice ( Oryza sativa ) grains using recombinant inbred lines derived from a cross between 'Zhenshan97B' and 'Nanyangzhan'
Author(s) -
Zhang Xiaona,
Li Pingbo,
Yun Peng,
Wang Lingqiang,
Gao Guanjun,
Zhang Qinglu,
Luo Lijun,
Zhang Chunyu,
He Yuqing
Publication year - 2019
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12739
Subject(s) - biology , quantitative trait locus , genetics , indel , oryza sativa , single nucleotide polymorphism , inbred strain , candidate gene , locus (genetics) , population , genetic linkage , gene , nonsynonymous substitution , genotype , genome , demography , sociology
Vitamin E (VE) is an important nutritional trait in rice grains. In order to dissect the genetic basis underlying VE content, a recombinant inbred lines population derived from 'Zhenshan 97B' and 'Nanyangzhan' was used for quantitative trait locus (QTL) analysis. Totally, 29 QTLs for six VE traits were identified in 2 consecutive years. Among those, five QTLs repeatedly detected in two years formed a cluster on chromosome 2, which was responsible for all five VE isomers. OsγTMT , the gene encoding γ‐tocopherol methyltransferase in rice, was located to the same region and treated as the candidate gene. Sequence analysis of alleles from two parents revealed many polymorphisms, including 19 single nucleotide polymorphisms (SNPs) and two insert/deletions (Indels) in the promoter region, two nonsynonymous SNPs in exons, and 25 SNPs and an Indel in introns. Besides, a QTL for δ‐tocotrienol and two QTLs for α‐tocopherol were repeatedly detected on chromosome 5 and 8, respectively, all three regions carrying no homologous genes involved in VE biosynthesis. These results could be useful in development of rice lines displaying desirable VE content.