Premium
Inheritance and molecular mapping of restorer‐of‐fertility (Rf) gene in A 2 hybrid system in pigeonpea ( Cajanus cajan )
Author(s) -
Sharma Pankaj,
Singh Inderjit,
Sirari Asmita,
Khosla Gaurav,
Singh Gurjeet,
Ludhar Navkiran Kaur,
Singh Sarvjeet
Publication year - 2019
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12737
Subject(s) - biology , cajanus , genetics , primer (cosmetics) , population , genotyping , bulked segregant analysis , marker assisted selection , gene , genetic marker , gene mapping , genotype , horticulture , chemistry , demography , organic chemistry , sociology , chromosome
Abstract Inheritance of fertility restorer gene in pigeonpea was studied using F 2 and BC 1 F 1 populations derived from cross AL103A × IC245273. It was found to be controlled by single dominant gene. Out of 228 SSR primer pairs, 33 primer pairs showed parental polymorphism, while four primers were found polymorphic in bulk segregant analysis (BSA). These four primers viz., CcM 1615, CcM 0710, CcM 0765 and CcM 1522 were used for genotyping of F 2 population and were found to be placed at 3.1, 5.1, 28.1 and 45.8 cM, respectively. Two of them, CcM 1615 and CcM 0710, evinced clear and unambiguous bands for fertility restoration in F 2 population. The Rf gene was mapped on linkage group 6 between the SSR markers CcM 1615 and CcM 0710 with the distances of 3.1 and 5.1 cM, respectively. The accuracy of the CcM 1615 was validated in 18 restorers and six maintainer lines. The marker CcM 1615 amplified in majority of male restorer lines with a selection accuracy of 91.66%.