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Separation and identification of Brassica rapa BrAFP and its gene cloning and expression under freezing stress
Author(s) -
Liu Zigang,
Dong Xiaoyun,
Ma Li,
Sun Wancang,
Yang Gang,
Fang Yan,
Wu Junyan,
Li Xuecai
Publication year - 2019
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12677
Subject(s) - brassica rapa , antifreeze protein , biology , brassica , ice crystals , gene , ice nucleus , gene expression , amino acid , cold shock domain , botany , thaumatin , microbiology and biotechnology , biochemistry , nucleation , chemistry , rna , physics , organic chemistry , optics
Antifreeze proteins (AFPs) are proteins that inhibit ice nucleation by non‐colligative combined with ice, to inhibit its growth and to modify ice crystal morphology. In this study, 'Longyou 6' ( Brassica rapa ) with the cold resistance was used as a model. A 38 kDa band of protein extracted from leaves of plants exposed to low temperature was enhanced on the electropherogram. The 38 kDa protein can significantly inhibit growth of ice crystals and modify its morphology, thus suggesting this protein has an AFP in winter turnip rape. The protein was identified as beta‐1,3‐glucanase by mass spectrometry, named BrAFP1. Its corresponding gene contains 1,032 bp bases, coding 343 amino acids with a molecular weight of 38,102 kDa. The BrAFP1 is a predicted hydrophobic protein with a signal peptide. Under cold stress, the plants showed morphological characteristics of cold resistance, and BrAFP expression was significantly up‐regulated at −4°C from 0.5 to 4 days compared with the control (at room temperature), suggesting that the BrAFP gene played an important role in cold acclimation in B. rapa .