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Variable resistance of bread wheat ( Triticum aestivum ) lines carrying 2 NS /2 AS translocation to wheat blast
Author(s) -
Cardozo Téllez Lourdes,
Chavez Alice,
Bobadilla Nathalia,
PérezEstigarribia Pastor,
Kohli Mohan
Publication year - 2019
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12661
Subject(s) - germplasm , biology , pyricularia , chromosomal translocation , cultivar , inoculation , poaceae , plant disease resistance , blast disease , agronomy , horticulture , botany , gene , genetics
Wheat blast disease, caused by Magnaporthe oryzae (anamorph Pyricularia oryzae ), produces severe damage to wheat production in South America. It was observed that many resistant cultivars contain the 2 NS /2 AS translocation from Triticum ventricosum . In this study, we evaluate the presence of the 2 NS /2 AS translocation in 57 advanced breeding lines and one variety ‘Caninde 1’ from Paraguayan wheat germplasm, using VENTRIUP ‐ LN 2 primers. The germplasm ‘Caninde 1 and 22’ of the breeding lines, found positive for the presence of 2 NS /2 AS translocation, were inoculated with a single aggressive Magnaporthe pathotype P14‐039, to assess their response to wheat blast infection under controlled conditions. Based on the disease infection score, ten of the breeding lines, ‘Caninde 1’ and ‘Milan’ (positive control), were classified as resistant. Three of the remaining breeding lines were classified as moderately resistant, five as moderately susceptible and other four as susceptible. Our results show that the expression of 2 NS /2 AS ‐based blast resistance is more dependent on genetic background of the inserted germplasm than previously envisioned.