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Pooled mapping of quantitative trait loci conferring male sterility‐conditioned glume split in rice ( Oryza sativa )
Author(s) -
Omboki Ratemo Billy,
Zheng Yan,
Chen Zhiwei,
Guan Huazhong,
Tang Weiqi,
Huang Likun,
Xie Xiaofang,
Wu Weiren
Publication year - 2018
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12643
Subject(s) - biology , bulked segregant analysis , glume , quantitative trait locus , sterility , oryza sativa , genetics , japonica , population , selfing , gene mapping , gene , botany , chromosome , demography , sociology
Glume split ( GS ) refers to the phenomenon that the lemma and palea of a rice seed cannot be fully closed. There is a kind of GS that occurs only under male sterility. Such male sterility‐conditioned GS (msc GS ) can severely reduce the quality of hybrid rice seeds and therefore is a serious problem in hybrid rice production. To reveal the genetic basis of msc GS , we employed the method of bulked segregant analysis combined with deep sequencing to map quantitative trait loci ( QTL s) conferring msc GS . By analysing a pair of opposite DNA pools made up of 50 extreme individuals each from an F 2 population of 509 individuals, we mapped two major QTL s on the long arm terminals of chromosomes 5 and 6, respectively. We named them qmsc GS 5 and qmsc GS 6 . The two QTL s both function mainly in additive mode, explaining a heritability of 16.98% and 11.96% by their additive effects, respectively. The information obtained in this study will facilitate marker‐assisted breeding of low‐ GS male‐sterile lines and positional cloning of genes controlling msc GS in rice.