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Genetic analysis of Fusarium wilt resistance in cowpea (V igna unguiculata Walp.)
Author(s) -
Omoigui Lucky O.,
Danmaigona Catherine C.,
Kamara Alpha Y.,
Ekefan Ebenezer J.,
Timko Michael P.
Publication year - 2018
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12628
Subject(s) - backcrossing , biology , fusarium wilt , inoculation , vigna , gene , fusarium oxysporum , inheritance (genetic algorithm) , horticulture , genetics , botany
This study investigated the inheritance of resistance to Fusarium oxysporum f.sp . tracheiphilum ( Fot ) in cowpea lines. Resistant and susceptible cowpea lines were crossed to develop F 1 , F 2 and backcross populations. Reaction to Fot was evaluated in 2015 and 2016 using seed soak and modified root‐dip inoculation methods. The expression of resistance reaction in the F 1 and segregation in F 2 generations indicated the role of dominant gene controlling Fot in cowpea. These results were further supported by the result of backcross ( BC 1 P 1 F 1 and BC 1 P 2 F 1 ) progeny tests. The backcross of F 1 with the resistant parent produced progeny that were uniformly resistant, whereas backcross of F 1 with the susceptible parent produced progeny that segregated into 1:1 ratio. The F 2 segregation ratio in the reciprocal cross showed no evidence of maternal effect in the inheritance of the resistance. Allelism test suggests that the gene for resistance in TV u 134 was the same in TV u 410 and TV u 109‐1. We also identified an SSR marker, C13‐16, that cosegregated with the gene conferring resistance to Fot in cowpea.