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Identification of quantitative trait loci for seed isoflavone concentration in soybean ( Glycine max ) against soybean cyst nematode stress
Author(s) -
Carter Adam,
Tenuta Albert,
Rajcan Istvan,
Welacky Tom,
Woodrow Lorna,
Eskandari Mehrzad
Publication year - 2018
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12627
Subject(s) - soybean cyst nematode , isoflavones , quantitative trait locus , biology , heterodera , population , glycine , genetics , cultivar , agronomy , gene , nematode , biochemistry , ecology , demography , amino acid , sociology
Abstract Isoflavones are plant secondary metabolites produced in soybean ( Glycine max ), which provide plant defense against pathogens and are beneficial to human health. Soybean cyst nematode (SCN) is a major yield‐limiting pest in most soybean‐producing area across the world. Traits, seed isoflavones and SCN resistance are quantitative in nature, and their phenotypic evaluations are expensive. Quantitative trait loci (QTL) underlying the two traits will be helpful to develop SCN‐resistant lines with elevated isoflavones using marker‐assisted‐selection (MAS). This study aims to identify isoflavones and SCN‐related QTL in a soybean population consisting of 109 RILs, which was developed from a cross between two commercial soybean cultivars viz. ‘RCAT1004’ and ‘DH4202’ and grown in four non‐SCN and SCN‐infested fields during 2015 and 2016. While single marker ANOVA identified 10 QTL for isoflavones and five for SCN ( p < 0.01), simple interval and multiple QTL mappings identified four QTL associated with isoflavones (LOD ≥ 2.2). These results contribute to a better understanding of the genetics of the two traits and provide molecular markers that can be used in MAS to facilitate developing SCN‐resistant soybeans with increased isoflavones.