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Mapping of quantitative trait loci associated with rice black‐streaked dwarf virus disease and its insect vector in rice ( Oryza sativa L.)
Author(s) -
Xu Tingting,
Liu Yuqiang,
Zhang Le,
Liu Linglong,
Wang Chunming,
Hu Jinlong,
Sun Zhiguang,
Pan Gen,
Xiao Shizhuo,
He Jun,
Huang Jie,
Qiu Zeyu,
Fan Dejia,
Jiang Ling,
Cheng Xianian,
Zhai Huqu,
Wan Jianmin
Publication year - 2018
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12620
Subject(s) - biology , brown planthopper , quantitative trait locus , japonica , oryza sativa , population , allele , plant disease resistance , cultivar , horticulture , veterinary medicine , genetics , botany , gene , medicine , demography , sociology
Abstract Rice black‐streaked dwarf virus disease ( RBSDVD ), transmitted by small brown planthopper ( SBPH , Laodelphax striatellus ), causes serious loss in rice production. Breeding resistant cultivars are one of the most effective strategies to control the virus disease and its vector. By both natural inoculations in the field and modified seedling‐box screening test in the glasshouse, an indica variety WR 24 showed high resistance to RBSDVD and SBPH . An F 2:3 population consisting of 153 lines derived from a cross between WR 24 and a susceptible japonica variety Suyunuo was used for quantitative trait loci ( QTL ) analysis of RBSDVD and SBPH resistance. The linkage map consisting of 130 SSR markers was constructed with an average marker interval of 13.90 cM , spanning a total of 1890.9 cM . Totally, five QTL s for RBSDV resistance, viz. qRBSDV 3 WR24 , qRBSDV 6 WR24 , qRBSDV 7 WR24 , qRBSDV 9 WR24 and qRBSDV 11 WR24 , were detected on chromosomes 3, 6, 7, 9 and 11, with LOD scores of 2.7, 3.08, 3.13, 5.28 and 3.7, respectively. Meanwhile, three QTL s for SBPH resistance, including qSBPH 5 WR24 , qSBPH 7 WR24 and qSBPH 10 WR24 , were mapped on chromosomes 5, 7 and 10, with LOD scores of 2.18, 3.5 and 3.57, respectively. All resistant alleles were from WR 24. Among these QTL s, qRBSDV 7 WR24 , qSBPH 5 WR24 and qSBPH 10 WR24 were newly reported, and qSBPH 10 WR24 showed major effect that explained 17.9% of total phenotypic variance. The RBSDVD and SBPH resistance QTL s and the tightly linked DNA markers can be utilized in RBSDV and SBPH resistance breeding in rice.