Premium
Breeding for boron tolerance in lentil ( Lens culinaris Medik.) using a high‐throughput phenotypic assay and molecular markers
Author(s) -
Rodda Matthew S.,
Sudheesh Shimna,
Javid Muhammad,
Noy Dianne,
Gnanasambandam Annathurai,
Slater Anthony T.,
Rosewarne Garry M.,
Kaur Sukhjiwan
Publication year - 2018
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12608
Subject(s) - biology , quantitative trait locus , germplasm , genetics , genetic marker , marker assisted selection , population , genetic linkage , inbred strain , locus (genetics) , trait , phenotype , molecular marker , gene mapping , gene , botany , chromosome , demography , sociology , computer science , programming language
This study describes the identification of a quantitative trait locus ( QTL ) in the recombinant inbred line population of ILL 2024 × ILL 6788 and subsequent validation of associated molecular markers. A high‐quality genetic linkage map was constructed with 758 markers that cover 1,057 cM, with an average intermarker distance of 2 cM. QTL analysis revealed a single genomic region on Lc2 to be associated with B tolerance and accounted for up to 76% of phenotypic variation (Vp). The best markers for B tolerance were assessed for their utility in routine breeding applications using validation panels of diverse lentil germplasm and breeding material derived from ILL 2024. A marker generated from the dense genetic map of this study was found to be the most accurate of all markers available for B tolerance in lentil, with a success rate of 93% within a large breeding pool derived from ILL 2024. However, given the number of the unrelated lines for which the marker–trait association was not conserved, B tolerance screening is still required at later stages to confirm predicted phenotypes.