Breeding for boron tolerance in lentil ( Lens culinaris  Medik.) using a high‐throughput phenotypic assay and molecular markers | Zendy

Rodda Matthew S. | Zendy; Sudheesh Shimna | Zendy; Javid Muhammad | Zendy; Noy Dianne | Zendy; Gnanasambandam Annathurai | Zendy; Slater Anthony T. | Zendy; Rosewarne Garry M. | Zendy; Kaur Sukhjiwan | Zendy

Premium

Breeding for boron tolerance in lentil ( Lens culinaris Medik.) using a high‐throughput phenotypic assay and molecular markers

Author(s) -

Rodda Matthew S.,

Sudheesh Shimna,

Javid Muhammad,

Noy Dianne,

Gnanasambandam Annathurai,

Slater Anthony T.,

Rosewarne Garry M.,

Kaur Sukhjiwan

Publication year - 2018

Publication title -

plant breeding

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.583

H-Index - 71

eISSN - 1439-0523

pISSN - 0179-9541

DOI - 10.1111/pbr.12608

Subject(s) - biology , quantitative trait locus , germplasm , genetics , genetic marker , marker assisted selection , population , genetic linkage , inbred strain , locus (genetics) , trait , phenotype , molecular marker , gene mapping , gene , botany , chromosome , demography , sociology , computer science , programming language

This study describes the identification of a quantitative trait locus ( QTL ) in the recombinant inbred line population of ILL 2024 × ILL 6788 and subsequent validation of associated molecular markers. A high‐quality genetic linkage map was constructed with 758 markers that cover 1,057 cM, with an average intermarker distance of 2 cM. QTL analysis revealed a single genomic region on Lc2 to be associated with B tolerance and accounted for up to 76% of phenotypic variation (Vp). The best markers for B tolerance were assessed for their utility in routine breeding applications using validation panels of diverse lentil germplasm and breeding material derived from ILL 2024. A marker generated from the dense genetic map of this study was found to be the most accurate of all markers available for B tolerance in lentil, with a success rate of 93% within a large breeding pool derived from ILL 2024. However, given the number of the unrelated lines for which the marker–trait association was not conserved, B tolerance screening is still required at later stages to confirm predicted phenotypes.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research