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Development of rice ( Oryza sativa ) lines resistant to aryloxyphenoxypropionate herbicides through induced mutation with gamma rays
Author(s) -
Andrade Alexander,
TulmannNeto Augusto,
Tcacenco Fernando A.,
Marschalek Rubens,
Pereira Adriana,
Oliveira Neto Antonio M.,
Scheuermann Klaus K.,
Wickert Ester,
Noldin José A.
Publication year - 2018
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12592
Subject(s) - oryza sativa , biology , weedy rice , acetyl coa carboxylase , oryza , herbicide resistance , mutation , point mutation , gene , pyruvate carboxylase , weed control , botany , agronomy , enzyme , genetics , biochemistry
The aryloxyphenoxypropionate herbicides ( APP s) are graminicides with excellent control of many grass weeds species, including weedy rice ( Oryza sativa L.). These herbicides block the fatty acid biosynthesis by inhibiting the enzyme acetyl‐CoA carboxylase ( ACC ase), resulting in the death of susceptible plants. Inducing mutation with gamma rays to rice seeds, two lines resistant to APP s herbicides were developed. Plant dose–response assays confirmed the resistance to the APPS herbicides quizalofop‐p‐ethyl and haloxyfop‐p‐methyl. The carboxyltransferase domain fragments of ACC ase from the resistant biotype and susceptible control were sequenced and compared. A point mutation was detected in the amino acid position 2,027 (Rice Genome Annotation Project: Os05g22940.1). Results indicated that resistance to APP s is a consequence of an altered ACC ase enzyme that confers resistance. The use of APP s herbicide‐resistant rice lines represents an innovative and promising alternative for weedy rice control in paddy rice systems.