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Fine mapping of Rf2 , a major locus controlling pollen fertility restoration in sorghum A 1 cytoplasm, encodes a PPR gene and its validation through expression analysis
Author(s) -
Madugula Praveen,
Uttam Anurag G.,
Tonapi Vilas A.,
Ragimasalawada Madhusudhana
Publication year - 2018
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12569
Subject(s) - cytoplasmic male sterility , biology , locus (genetics) , genetics , gene , fertility , sorghum , agronomy , population , demography , sociology
Abstract Sorghum is one of the pioneering cereal crops where cytoplasmic male sterility ( CMS ) was successfully exploited for mass production of F 1 hybrid seed. Mapping genes for fertility restoration ( Rf ) is an important aspect of understanding the molecular basis of fertility restoration in crop plants. In this study, we fine‐mapped a fertility restoration locus, Rf2 of sorghum reported earlier (Jordan, Mace, Henzell, Klein, & Klein, [Jordan, D., 2010]), involving two F 2 populations (296A × RS 29 and 296A × DSV 1) and newly developed SSR markers delimited Rf2 locus to 10.32‐kb region on chromosome 2. The Rf2 locus was tightly linked with two new SSR s, MS ‐ SB 02‐3460 (0.14 cM ) and MS ‐ SB 02‐3466 (0.75 cM ) on both sides, and hosted only one gene ( Sobic.002G057050 ) of PPR gene family. Another new SSR marker developed in the study, MS ‐ SB 02‐37912, forms the part of PPR gene and could act as a perfect marker in marker‐assisted breeding for fertility restoration involving Rf2 in sorghum breeding. The strong involvement of Sobic.002G057050 gene in fertility restoration was supported through RNA expression analysis.