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Production of a desirable Brassica oleracea CMS line using an alloplasmic B. rapa CMS line carrying Diplotaxis erucoides cytoplasm as a bridge plant
Author(s) -
Fujita Yoshiaki,
Sunaga Keita,
Shim SuHyeun,
Yamada Wataru,
Ohnishi Takayuki,
Bang Sang Woo
Publication year - 2018
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12565
Subject(s) - brassica oleracea , cytoplasmic male sterility , biology , backcrossing , hybrid seed , brassica rapa , pollen , stamen , doubled haploidy , botany , cytoplasm , sterility , cultivar , horticulture , hybrid , ploidy , brassica , microbiology and biotechnology , gene , genetics
In Brassica oleracea , production of F 1 hybrid seeds mainly makes use of the improved Ogura cytoplasmic male sterile ( CMS ) line. However, reliance on one particular line is a risk, and it would be advantageous to develop other CMS lines. In this study, we transferred Diplotaxis erucoides cytoplasm to B. oleracea cultivars using an alloplasmic B. rapa CMS line as a bridge plant to avoid incompatibility between donor and recipient plants. The new B. oleracea CMS lines, which were derived by four generations of backcrossing, had small rudimentary anthers with no pollen grain and showed complete male sterility. There was no functional defect in other floral organs, and the ability to receive normal pollen did not appear to be impaired. Moreover, the B. oleracea CMS lines carrying D. erucoides cytoplasm had larger leaf areas and a normal plastochron. As a consequence, the B. oleracea CMS lines carrying D. erucoides cytoplasm have the potential to be valuable alternatives for use in commercial B. oleracea hybrid seed production.