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Development of a molecular marker for self‐compatible S4′ haplotype in sweet cherry ( Prunus avium L.) using high‐resolution melting
Author(s) -
MuñozEspinoza Claudia,
Espinosa Evelyn,
Bascuñán Roberto,
Tapia Sebastián,
Meneses Claudio,
Almeida Andréa Miyasaka
Publication year - 2017
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12546
Subject(s) - biology , prunus , genetics , allele , molecular marker , haplotype , genetic marker , marker assisted selection , locus (genetics) , gene , botany
Sweet cherry ( Prunus avium L.) has stylar gametophytic self‐incompatibility, which is controlled by the multi‐allelic S ‐locus and encompasses the highly polymorphic genes for the S ‐ribonuclease ( S ‐RNase) and S ‐haplotype‐specific F‐box ( SFB ), which are female and male determinants, respectively. The self‐compatible mutant SFB 4′ corresponds to an allele variant of SFB 4 and presents a frameshift mutation. Even though male‐determinant molecular markers can discriminate between SFB 4 and SFB 4′ alleles, the methods required are laborious, time‐consuming and expensive, and not suitable for massive analysis and integration into breeding programmes. Our aim was to develop molecular markers for the evaluation of self‐compatibility alleles in sweet cherry, that could be used as a high‐throughput screening strategy to identify SFB 4 and SFB 4′ alleles, based on a marker for male determinacy. Our results were consistent using primers flanking the mutation responsible for the SFB 4′ allele. We designed a specific molecular marker and confirmed it in sweet cherry commercial varieties. This new molecular marker is feasible for self‐compatibility alleles in the male determinant in sweet cherry‐assisted breeding programs.