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Identification of potential gene‐associated major traits using GBS ‐ GWAS for Korean apple germplasm collections
Author(s) -
Lee So Jin,
Ban Seung Hyun,
Kim Gi Hoon,
Kwon Soon Il,
Kim Jeong Hee,
Choi Cheol
Publication year - 2017
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12544
Subject(s) - genome wide association study , germplasm , biology , single nucleotide polymorphism , genetics , genetic association , genotyping , snp , identification (biology) , computational biology , gene , genotype , botany
Abstract Genomewide association study ( GWAS ), which queries the association between loci and a particular trait by examining single nucleotide polymorphisms ( SNP s) of the entire genome, is used in many fields of study. The development of next‐generation sequencing techniques has facilitated GWAS s by decreasing the sequencing costs and time. In particular, genotyping by sequencing ( GBS ) is useful for sequencing many samples simultaneously and at a moderate price. Herein, we describe a potential GWAS using GBS , focused on the apple germplasm, with the goal of developing an effective apple breeding strategy through the identification of useful markers. From 308 Korean apple germplasm, SNP s were selected after GBS , and major traits were investigated. Proprietary individuals were confirmed and grouped by association through genetic diversity and population structure analyses of the selected SNP s. Genes highly associated with the target traits were identified, respectively. As the first GWAS report on the apple germplasm, these results will be useful as base data for GWAS s on other apple populations and traits.