Premium
QTL analysis for alkaline tolerance of rice and verification of a major QTL
Author(s) -
Li Ning,
Sun Jian,
Wang Jingguo,
Liu Hualong,
Zheng Hongliang,
Yang Luomiao,
Liang Yingpei,
Li Xianwei,
Zou Detang
Publication year - 2017
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12539
Subject(s) - quantitative trait locus , biology , population , shoot , gene , genetics , horticulture , botany , demography , sociology
Alkaline stress causes injuries to rice seedlings in many parts of the world and is therefore an important factor affecting rice production in such areas. In this study, we preliminarily located quantitative trait loci ( QTL s) for survival days of seedlings ( SDS ) and the concentrations of Na + and K + in shoots ( SNC and SKC ) and roots ( RNC and RKC ) under alkaline stress using an F 2:3 population, which was derived from a cross between Caidao and WD 20342 with 151 simple sequence repeat ( SSR ) markers. A total of seven QTL s were detected. Of these QTL s, qSNC 3 had the largest effect, which explained 21.24% of the total phenotypic and is a major QTL . Next, a mapping population consisting of 190 BC 2 F 2 plants was constructed using WD 20342 as a donor parent to verify qSNC 3 . As a result, qSNC 3 was delimited to an 81.7‐kb region between markers RM 1221 and RM 4404. In this region, LOC _Os03 g62500 and LOC _Os03 g62620 exhibited different expression between Caidao and WD 20342 under alkaline stress. These results provide a basis for identifying genes related to alkaline tolerance in rice.