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Molecular mapping and candidate gene analysis for fruit epidermal structure in cucumber
Author(s) -
Zhang Song,
Miao Han,
Song Zichao,
Liu Panna,
Wang Ye,
Wehner Todd C.,
Gu Xingfang,
Zhang Shengping
Publication year - 2017
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12510
Subject(s) - biology , epidermis (zoology) , candidate gene , genetics , quantitative trait locus , gene , gene mapping , botany , chromosome , anatomy
Firmness of skin is an important quality of processing cucumbers, which is a feature of the palisade epidermis in these types compared to flat epidermis in fresh cucumbers. This study was conducted to map the Pe (palisade epidermis) and analyse the candidate gene. Populations derived from the cross of two inbred lines, NCG 122 with fruit flat epidermis and NCG 121 with fruit palisade epidermis, were used to identify the inheritance of palisade epidermis and to map the gene involved in its development. The results showed that the palisade epidermis trait is controlled by a single gene, Pe , that is dominant over flat epidermis. Seven simple sequence repeat ( SSR ) and five insertion deletion (Indel) markers were identified to be linked to the Pe gene. It was mapped to cucumber chromosome 5 (Chr.5) between SSR 14611 and Indelpe12 with genetic distances of 0.3 cM and 0.2 cM, respectively. The physical distance of the genomic region harbouring the gene was 227.5 kb with 26 predicted candidate genes. The accuracy of marker‐assisted selection using the molecular markers, Indelpe12 and SSR 14611, was 68% and 88%, respectively.