Premium
Development of a cytoplasmic male‐sterile line NJCMS 4A for hybrid soybean production
Author(s) -
Nie Zhixing,
Zhao Tuanjie,
Yang Shouping,
Gai Junyi
Publication year - 2017
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12488
Subject(s) - biology , backcrossing , cytoplasmic male sterility , software maintainer , hybrid seed , sterility , glycine soja , glycine , cytoplasm , gene , botany , horticulture , genetics , microbiology and biotechnology , hybrid , amino acid
Cytoplasmic male‐sterile ( CMS ) lines are being used to produce hybrid seeds. Thus far, four CMS sources in soybean [ Glycine max (L.) Merr.] have been reported in China. However, they are not sufficient or efficient in meeting the requirements of commercial soybean hybrid seed production. In this study, 33 varieties were tested for CMS using 45 crosses among 37 landraces and 17 annual wild soybean accessions ( Glycine soja Sieb. et Zucc.). The cross of N23661 × N23658 showed partial to complete male sterility in backcross generations, while the corresponding reciprocal cross showed normal male fertility. Thus, the cytoplasm of N23661 is male‐sterile, the continuously backcrossed line is a male‐sterile line (designated NJCMS 4A), and N23658 is its maintainer (designated NJCM 4B). The male fertility of NJCMS 4A was restored by another accession, Nansheng9403. Accordingly, NJCMS 4A along with its maintainer and restorer composes a complete set of three lines for producing hybrid soybean. Using mitochondrial markers and sequence analyses, NJCMS 4A is a CMS line with its cytoplasm not identical to the four previously reported CMS sources in soybean.