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Validation of a set of informative simple sequence repeats markers for variety identification in Pak‐choi ( Brassica rapa L. ssp. chinensis var. communis )
Author(s) -
Su Tongbing,
Zhao Xiuyun,
Sui Guanglei,
Yu Shuancang,
Zhang Fenglan,
Wang Weihong,
Zhang Deshuang,
Yu Yangjun
Publication year - 2017
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12483
Subject(s) - biology , brassica rapa , microsatellite , genetic diversity , loss of heterozygosity , dendrogram , fixation index , genetics , brassica , germplasm , inbred strain , allele , botany , population , gene , demography , sociology
Abstract A core set of 21 simple sequence repeats ( SSR ) markers was developed for Pak‐choi ( Brassica rapa ssp. chinensis var. communis ) variety identification. We initially selected 74 SSR markers which exhibited high polymorphism and reproducibility in SSR detection from 2129 SSR s. Using the 74 SSR ‐based dendrogram for 45 inbred lines as calibration, 21 core SSR s were selected out. The utility of this core set SSR s was firstly tested in 45 inbred lines and finally verified in 102 commercial varieties. We also constructed a molecular ladder for each core SSR as a reference standard. Diversity analysis of this core SSR panel in 102 varieties demonstrated that each marker generates 2–3 alleles (averaged 2.33), with polymorphism information content values ranging from 0.01 to 0.56 (averaged 0.31). The averaged values of Shannon information index, observed heterozygosity, expected heterozygosity and Wright's fixation index were 0.59, 0.43, 0.38 and −0.09, respectively. Furthermore, the 21 SSR ‐based classifications for 102 varieties were consistent with traditional classification based on morphology. This core SSR panel represents an effective tool for genetic variation analysis in Pak‐choi.