Premium
Genetic diversity of garlic ( Allium sativum L.) germplasm from China by fluorescent‐based AFLP , SSR and InDel markers
Author(s) -
Wang Haiping,
Li Xixiang,
Liu Xiuhui,
Oiu Yang,
Song Jiangping,
Zhang Xiaohui
Publication year - 2016
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12424
Subject(s) - amplified fragment length polymorphism , biology , germplasm , allium sativum , indel , genetic diversity , population , microsatellite , botany , genetics , allele , genotype , single nucleotide polymorphism , gene , demography , sociology
Garlic ( Allium sativum L.), an asexually propagated crop, is an important vegetable and medicinal plant. China is the biggest garlic producer in the world; however, the genetic background of garlic from China is not well understood. In this study, population structure and clustering analysis of garlic germplasm was performed using amplified fragment length polymorphism ( AFLP ), simple sequence repeat ( SSR ) and insertion–deletion (InDel) markers. Among 212 accessions of garlic, genetic diversity analysis identified 546 alleles amplified by AFLP , SSR and InDel primers, and 492 of these were polymorphic. All accessions were divided into five groups by structure analysis and neighbor‐joining clustering. Most traits, including allicin content, were only slightly affected by population structure, which indicated that this germplasm can be used as populations for association mapping. The results provide a molecular basis for understanding the genetic diversity of the garlic germplasm preserved in China.