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Mapping of male sterility gene ms10 in chilli pepper ( Capsicum annuum L.)
Author(s) -
Aulakh Parjeet Singh,
Dhaliwal Major S.,
Jindal Salesh Kumar,
Schafleitner Roland,
Singh Kuldeep
Publication year - 2016
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12389
Subject(s) - biology , bulked segregant analysis , sterility , genetics , gene , marker assisted selection , pepper , cytoplasmic male sterility , gene mapping , capsicum annuum , genetic marker , genetic linkage , hybrid seed , primer (cosmetics) , hybrid , molecular marker , marker gene , chromosome , horticulture , chemistry , organic chemistry
Most of the hybrid seed in chilli are produced manually, but the use of male sterility ( MS ) can reduce the cost of hybrid seed production. MS ‐12, a nuclear male‐sterile ( NMS ) line developed at Punjab Agricultural University, Ludhiana (India), has been utilized to develop commercial F 1 hybrids. A recessive gene, designated as ms10 , governs MS in MS ‐12. Due to recessive gene control, development of new NMS lines incorporating ms10 gene is tedious and time‐consuming. We identified SSR markers AVRDC ‐ PP 12 and AVRDC _ MD 997* linked to the ms10 gene. A total of 558 primer pairs were screened following bulked segregant analysis ( BSA ). Linkage analysis in 210 F 2 plants indicated that the two SSR markers were linked to the ms10 gene and the marker AVRDC ‐ PP 12 was closest to the gene at 7.2 cM distance. The marker was mapped to chromosome 1 at genome position 175 694 513 to 175 694 644. Until more closely linked markers are developed, the marker AVRDC ‐ PP 12 would facilitate transfer of ms10 gene through marker‐assisted selection ( MAS ). Fine mapping would lead to cloning of the ms10 gene.