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Genomic affinities between Brassica napus and Raphanu s raphanistrum as revealed by meiotic GISH
Author(s) -
Singh Satinder,
Kaur Gurpreet,
Gupta Mehak,
Banga Shashi,
Banga S. S.
Publication year - 2016
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12384
Subject(s) - biology , introgression , brassica , meiosis , genetics , genome , bivalent (engine) , hybrid , pollen , botany , gene , chemistry , organic chemistry , metal
An intergeneric hybrid between Brassica napus ( AC , n = 19) and Raphanus raphanistrum (Rr, n = 9) was developed using the sequential ovary–ovule culture. Morphological, molecular, cytological studies and fluorescent genomic in situ hybridization were conducted to establish the hybridity and genomic relatedness. F 1 hybrid plants were male sterile and morphologically intermediate between the two parents. Cytological analysis of the hybrid plants revealed 10 II + 8I as the predominant meiotic configuration with the mean bivalent frequency of 8.83. A maximum of 13 II were observed in 8.24% of the pollen mother cells ( PMC s). GISH studies facilitated the identification of two allopairs, confirming homoeologous pairing between A/C and Rr genomes. This may have significant implications in terms of transgene flow and possible introgression into R . raphanistrum , which is a common canola weed in Canada and Australia. The intergeneric hybrid B . napus × R . raphanistrum so produced also has the potential to be used as a bridging species for the transfer of desirable genes to amphiploid crop Brassica species carrying A/C genomes.