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Development of gene‐based markers for the Turnip mosaic virus resistance gene retr02 in Brassica rapa
Author(s) -
Li GuoLiang,
Qian Wei,
Zhang ShuJiang,
Zhang ShiFan,
Li Fei,
Zhang Hui,
Wu Jian,
Wang XiaoWu,
Sun RiFei
Publication year - 2016
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12372
Subject(s) - biology , brassica rapa , genetics , marker assisted selection , gene , molecular marker , genetic marker , variants of pcr , cleaved amplified polymorphic sequence , marker gene , allele , brassica , clubroot , sequence tagged site , exon , polymerase chain reaction , botany , restriction fragment length polymorphism , gene mapping , chromosome
Turnip mosaic virus (Tu MV ) is responsible for a serious disease that affects the production of Chinese cabbage. Previous studies have cloned a series of Tu MV resistance genes and developed molecular markers. In this study, a derived cleaved amplified polymorphism sequence ( dCAPS ) marker and a Kompetitive Allele Specific PCR ( KASP ) marker were developed based on a single recessive gene, retr02 , which confers broad‐spectrum Tu MV resistance in Chinese cabbage by means of an additional G at the junction of exon 1 and intron 1. The two markers were able to detect the retr02 allele in Chinese cabbage accessions used in breeding programmes. Compared with the dCAPS marker, the KASP marker was flexible, cost‐effective and quick to process, which is likely to be beneficial in establishing high‐throughput assays for marker‐assisted selection.