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Generation of tribenuron‐methyl herbicide‐resistant Os CYP 81A6 ‐expressing rapeseed ( Brassica napus L.) plants for hybrid seed production using chemical‐induced male sterility
Author(s) -
Yao Xuan,
Wang Yaqin,
Yue Xiaopeng,
Liu Meng,
Liu Kede
Publication year - 2016
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12361
Subject(s) - rapeseed , sterility , brassica , biology , sulfonylurea , hybrid seed , bentazon , agronomy , horticulture , botany , microbiology and biotechnology , hybrid , weed , insulin
Chemical‐induced male sterility ( CIMS ) is a method for hybrid rapeseed ( Brassica napus L.) production. Some sulphonylurea herbicides such as tribenuron‐methyl ( TBM ) are used as chemical hybridization agents ( CHA s) in CIMS systems. However, the male parents must be protected from herbicide injury with a shield during spraying of the female parents with CHA s to induce male sterility. Thus, using herbicide‐resistant rapeseed lines as the male parents can significantly simplify the seed production procedure and reduce the cost in hybrid seed production. A rice cytochrome P450 hydroxylase, Os CYP 81A6, has been previously characterized to confer resistance to bentazon and sulphonylurea herbicides. We demonstrate here that the introduction of Os CYP 81A6 renders rapeseed plants resistant to TBM . Compared with wild‐type plants, the transgenic plants displayed normal stamen development and male fertility when treated with 0.05 mg/l of TBM , the dose used for inducing male sterility in hybrid seed production. These results indicate that the Os CYP 81A6 ‐expressing rapeseed plants can be used as the male parents for hybrid rapeseed production using CIMS .