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A Robertsonian translocation from Thinopyrum bessarabicum into bread wheat confers high iron and zinc contents
Author(s) -
Ardalani Somayeh,
Mirzaghaderi Ghader,
Badakhshan Hedyeh
Publication year - 2016
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12359
Subject(s) - biology , chromosomal translocation , robertsonian translocation , population , cultivar , chromosome , common wheat , ploidy , doubled haploidy , genetics , gene , botany , karyotype , demography , sociology
Development of wheat–alien translocation lines has facilitated practical utilization of alien species in wheat improvement. The production of a compensating Triticum aestivum – Thinopyrum bessarabicum whole‐arm Robertsonian translocation (RobT) involving chromosomes 6D of wheat and 6E b of Th. bessarabicum (2n = 2 x = 14, E b E b ) through the mechanism of centric breakage–fusion is reported here. An F 2 population was derived from plants double‐monosomic for chromosome 6D and 6E b from crosses between a DS 6E b (6D) substitution line and bread wheat cultivar ‘Roushan’ (2n = 6 x = 42, AABBDD ) as female parent. Eighty F 2 genotypes (L1–L80) were screened for chromosome composition. Three PCR ‐based Landmark Unique Gene ( PLUG ) markers specific to chromosomes 6D and 6E b were used for screening the F 2 plants. One plant with a T6E b S.6 DL centric fusion (RobT) was identified. A homozygous translocation line with full fertility was recovered among F 3 families and verified with genomic in situ hybridization ( GISH ). Grain micronutrient analysis showed that the DS 6E b (6D) substitution line and T6E b S.6 DL stock have higher Fe and Zn contents than the recipient wheat cultivar ‘Roushan’.