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Dynamic quantitative trait loci underlies isoflavone accumulation in soybean seed
Author(s) -
Han Yingpeng,
Li Dongmei,
Zhao Guiyun,
Zhao Xue,
Jiang Zhenfeng,
Hu Haibo,
Wu Lin,
Wang Yan,
Gao Yuan,
Li Yongguang,
Zeng Guoliang,
Meng Fanli,
Teng Weili,
Li Wenbin
Publication year - 2016
Publication title -
plant breeding
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.583
H-Index - 71
eISSN - 1439-0523
pISSN - 0179-9541
DOI - 10.1111/pbr.12354
Subject(s) - quantitative trait locus , biology , glycitein , daidzein , genetics , genistein , gene
Most of quantitative trait loci ( QTL ) underlying soybean seed isoflavone contents were derived from the harvest stage of plant development, which uncover the genetic effects that were expressed in earlier seed developmental stages. The aim of this study was to detect conditional QTL associated with isoflavone accumulation during the entire seed development. A total of 112 recombinant inbred lines developed from the cross between ‘Zhongdou 27’ (higher seed isoflavone content) and ‘Jiunong 20’ (lower seed isoflavone content) were used for the conditional QTL analysis of daidzein ( DZ ), genistein ( GT ), glycitein ( GC ) and total isoflavone ( TI ) accumulations through composite interval mapping with mixed genetic model. The results indicated that the number and type of QTL and their additive effects for individual and total isoflavone accumulations were different among R3 to R8 developmental stages. Three unconditional QTL and six conditional QTL for DZ , four unconditional QTL and five conditional QTL for GT , six unconditional QTL and five conditional QTL for GC , six unconditional QTL and seven conditional QTL for TI were identified at different developmental stages, respectively. Unconditional and conditional QTL that affect individual and total isoflavone accumulations exhibited multiple expression patterns, implying that some QTL are active for long period and others are transient. Two genomic regions, Satt144‐Satt569 in linkage group F ( LG F; chromosome 13, chr 13) for DZ , GC , GT and TI accumulations and Satt540‐Sat_240 in LG M (chr 07) for TI and GC accumulations, were found to significantly affect individual and total isoflavone accumulations in multiple developmental stages, suggesting that the accumulation of soybean seed isoflavones is governed by time‐dependent gene expression.